TheAquaScreen®qPCRDetectionkitseriesisspecificallydesignedforthequantitativedetectionofcommonwaterpathogenssuchasLegionella,Pseudomonas,andE.coli.TheAquaScreen®approachincludessamplepreparationusingtheAquaScreen®FastExtractkitandvariousqPCRdetectionkits.Incontrasttotime-consumingcellculturemethods,theAquaScreen®approachneedslessthansixhoursincludingsamplepreparationandqPCRtoreliablydetectwaterpathogens.Inaddition,ourqPCRassayissuperiorintermsofsensitivityandspecificityastheassayonlydetectstherespectivespecies.TheAquaScreen®qPCRassayisinsensitivetocontaminationwithotherbacterialgenera.Also,theassay’srobustnessisunsurpassedwithlineardetectionupto3x106particlespersample.
- ➘AquaScreen®Legionellapneumophila
- ➘AquaScreen®Legionellaspecies
- ➘AquaScreen®Pseudomonasaeruginosa
- ➘AquaScreen®Escherichiacoli
AquaScreen®Legionellapneumophila
Features
TypeofPCR
Quantitativereal-timePCR(qPCR)
Description
TheAquaScreen®LegionellapneumophilaqPCRDetectionkitisusedforDNAsamplespreparedwiththeAquaScreen®FastExtractkitforquantificationofLegionellapneumophilainwaterssamples.TheusedprimersetisspecificforasegmentofthemipregionoftheLegionellapneumophilagenome.
ScopeofApplication
ApplicableinresearchandindustryforQAtestingofhouseholdandprocesswater.Notrecommendedforclinicaldiagnostics,testingofhumansamplesorpharmaceuticalproducts.
KitComponents
Freeze-driedPCRmixwithprimer,probes,nucleotides,andpolymerase
RehydrationBuffer
Freeze-driedPositiveControlDNA
Freeze-driedInternalAmplificationControl
PCR-gradeWater
PackageSizes
- Cat.No.34-202525reactions
Cat.No.34-2100100reactions
Cat.No.34-2250250reactions
RequiredConsumables
PCRreactiontubes
Optional:ForquantificationwerecommendourLegionellapneumophilaPCRQuantificationStandard(Cat.No.52-0101).
RequiredLabEquipment
qPCRcyclerwithfiltersetsforFAM™andROX™
Pipetsandfiltertips
Storage
Theunopenedkitshouldbestoredat+2to+8°C.Afterrehydrationthereagentsmustbestoredbelow-18°C.
Compliance
AFNORXPT90-471andISO/TS12869:2012
AquaScreen®Legionellaspecies
Features
TypeofPCR
Quantitativereal-timePCR(qPCR)
Description
TheAquaScreen®LegionellaspeciesqPCRDetectionKitisusedforDNAsamplespreparedwiththeAquaScreen®FastExtractkitforquantificationofLegionellainwaterssamples.Thesuppliedprimersetisspecificforabroadrangeoflegionellaspecies,butdoesnotdetectotherwaterbornebacteriaasrequiredbyISO/TS12869:2012.
ScopeofApplication
ApplicableforwatertestingasdescribedinISO/TS12869:2012,inresearchandindustryforQAtestingofprocesswater.Notrecommendedforclinicaldiagnostics,testingofhumansamplesorpharmaceuticalproducts.
KitComponents
Freeze-driedPCRmixwithprimer,probes,nucleotides,andpolymerase
RehydrationBuffer
Freeze-driedPositiveControlDNA
Freeze-driedInternalAmplificationControl
PCR-gradeWater
PackageSizes
- Cat.No.33-202525reactions
Cat.No.33-2100100reactions
Cat.No.33-2250250reactions
RequiredConsumables
PCRreactiontubes
Optional:ForquantificationwerecommendourLegionellapneumophilaPCRQuantificationStandard(Cat.No.52-0101).
RequiredLabEquipment
qPCRcyclerwithfiltersetsforFAM™andROX™
Pipetsandfiltertips
Storage
Theunopenedkitshouldbestoredat+2to+8°C.Afterrehydrationthereagentsmustbestoredbelow -18°C.
Compliance
AFNORXPT90-471andISO/TS12869:2012
AquaScreen®Pseudomonasaeruginosa
Features
TypeofPCR
Quantitativereal-timePCR(qPCR)
Description
TheAquaScreen®PseudomonasaeruginosaqPCRDetectionkitisusedforDNAsamplespreparedwiththeAquaScreen®FastExtractkitforquantificationofPseudomonasaeruginosainwaterssamples.
ScopeofApplication
ApplicableinresearchandindustryforQAtestingofhouseholdandprocesswater.Notrecommendedforclinicaldiagnostics,testingofhumansamplesorpharmaceuticalproducts.
KitComponents
Freeze-driedPCRmixwithprimer,probes,nucleotides,andpolymerase
RehydrationBuffer
Freeze-driedPositiveControlDNA
Freeze-driedInternalAmplificationControl
PCR-gradeWater
PackageSizes
- Cat.No.34-602525reactions
Cat.No.34-6100100reactions
Cat.No.34-6250250reactions
RequiredConsumables
PCRreactiontubes
Optional:ForquantificationwerecommendourPseudomonasaeruginosaPCRQuantificationStandard(Cat.No.52-0071).
RequiredLabEquipment
qPCRcyclerwithfiltersetsforFAM™andROX™
Pipetsandfiltertips
Storage
Theunopenedkitshouldbestoredat+2to+8°C.Afterrehydrationthereagentsmustbestoredbelow -18°C.
AquaScreen®Escherichiacoli
Features
TypeofPCR
Quantitativereal-timePCR(qPCR)
Description
TheAquaScreen®EscherichiacoliqPCRDetectionkitisusedforDNAsamplespreparedwiththeAquaScreen®FastExtractkitforquantificationofEscherichiacoliinwaterssamples.
ScopeofApplication
ApplicableinresearchandindustryforQAtestingofhouseholdandprocesswater.Notrecommendedforclinicaldiagnostics,testingofhumansamplesorpharmaceuticalproducts.
KitComponents
Freeze-driedPCRmixwithprimer,probes,nucleotides,andpolymerase
RehydrationBuffer
Freeze-driedPositiveControlDNA
Freeze-driedInternalAmplificationControl
PCR-gradeWater
PackageSizes
- Cat.No.34-702525reactions
Cat.No.34-7100100reactions
Cat.No.34-7250250reactions
RequiredConsumables
PCRreactiontubes
Optional:ForquantificationwerecommendourE.coliPCRQuantificationStandard(Cat.No.52-0083).
RequiredLabEquipment
qPCRcyclerwithfiltersetsforFAM™andROX™
Pipetsandfiltertips
Storage
Theunopenedkitshouldbestoredat+2to+8°C.Afterrehydrationthereagentsmustbestoredbelow -18°C.
