Thenumberofvegetariansandpeoplefollowingtheveganlifestyleincreasesfromyeartoyear.Thismaybeduetoethical,religious,culturalorhealthreasons.Howdoyouensurethatyourvegan-declaredfood has really beenprocessedwithoutmeat oranimalderivedproducts?
The VeganControl™kit,aqPCRbasedsystemforthedetectionofanimaltracesinfood,isaquickandinexpensivesolutiontothisProblem.
TypeofPCR
TaqMan®assaybasedonFAM™andHEX™labeledprobes.
RecommendedUse/Scope
Canbeusedinresearchandindustryfordetectionofmeat/animalbasedcontaminationsofveganfoods.Forresearchuseonly!Donotuseforclinicaldiagnosticorfor testingofpatientsamples.
PackageSizes
- Cat.Nr.12-05-02525Tests
Cat.Nr.12-05-100100Tests
ResultEvaluation
Cyclerbased,real-timePCR
Sensitivity
Downto0.1%ofgenomicmeattargetDNAinaveganfoodtypebackground.
Content
Primersets,nucleotides,internalamplificationcontrolandpolymeraseatoptimizedconcentrationslyophilizedinonetube,rehydrationbuffer,lyophilizedpositivecontrol,PCRgradewater.
SampleRequirements
IsolatedtotalDNAfrompotentiallycontaminatedveganfoodservesasstartingmaterial.DNAcanbeextractedfromsamplematerialusingDNAextraktionkitse.g.ExtractNow™VeganControl(Cat.No.607-1050)orusermethod.
RequiredConsumables
1.5mlreactiontubes,PCRreactiontubes,sterilefiltertips
RequiredLabDevices
qPCRcyclerwithFAM™andHEX™filter,variablemicroliterPipettes,benchtopcentrifuge
Timetoresult
Approx.150minutes
ShelfLifeandStorage
Componentscanbestoredat+2to+8°Cforatleast12months.Afterrehydratisationthereagentsmustbestoredat-18°C.