MeatID™HalalisakitforthefastandreliableidentificationofporkanddonkeyinmeatandotherfoodsbyqPCR.
Theidentificationofdifferentmeats,especiallyinmincedmeatproductsisaserioustaskinfoodsafetyandfromandethicalperspective,especiallyforMuslims.Authenticationofforbiddenornonedeclaredingredientssuchasporkordonkeymeatisessentialtoensureconfidenceinthesupplychainandregulatorycompliance.
MeatID™Halalisavailableforrapidandreliableanalysisfromvariousmatricesincludingraw,orevenhighlyprocessedandcookedmeatproductswheretheDNAmaybesignificantlydegraded.ItispossIBLetoidentifyrelevantspeciesdowntoathresholdlevelof0.5%withasemi-quantitativeresult.
TypeofPCR
TaqMan®AssaybasedonFAM™andHEX™labeledprobes.
RecommendedUse/Scope
CanbeusedinresearchandindustryfordetectionofporkanddonkeyinmeatandotherfoodsbyqPCR.Forresearchuseonly!Donotuseforclinicaldiagnosticortestingofpatientsamples.
Targetsequenceisamitochondrialgene(cytochromeb),whichisavailableinmultiplecopiespercell,enablingsuccessfuldetectionofminuteamountsoftargetDNA!
PackageSizes
- Cat.No.12-02-02525tests
Cat.No.12-02-100100Tests
ResultEvaluation
Cyclerbased,real-timePCR
Sensitivity
Downtoathresholdlevelof0.5%withasemi-quantitativeresult.
Content
Primersets,nucleotides,polymeraseandinternalcontrolDNA,lyophilized,filledinasingletube.Rehydrationbuffer,Positivecontrol,PCRgradewater.
SampleRequirements
DNAcanbeextractedfromsamplematerialusingDNAextractionkitse.g.ExtractNow™DNAMiniKit(Art.-Nr.608-1050)orusermethod.
RequiredConsumables
Pipettetips,1.5mlreactiontubes,PCRreactiontubes
RequiredLabDevices
qPCRcyclerwithFAM™andHEX™filter,benchtopcentrifuge,racksfortubes,variablemicroliterpipettes
Timetoresult
Approx.90minutes
ShelfLifeandStorage
Componentscanbestoredat+2to+8°Cforatleast12months.Afterrehydrationreagentsmustbestoredat-18°C.