OurPCRQuantificationStandardscontaingenomicDNAextractedfromlowpassageofdefinedmicroorganisms.TheDNAstartingmaterialwasanalyzedwithstandardizedmethodstodeterminetheDNA’sintegrityandquantity.OnlypureandintactDNAshowinga>10kbbandinanagarosegelandanOD260/280>1.8isused.TheDNAwaspartiallysequencedtoconfirmthespecies’identity.
ScopeofApplication
PCRQuantificationStandardscontainadefinednumberofgenomecopiesofaparticularmicroorganism.Therefore,ourstandardsareperfectlysuitableforgeneratingDNAstandardcurvesinqPCR.Inaddition,standardsarerequiredfortestingthespecificityandsensitivityofPCRassays,particularly,inthecontextofqualitycontrolandincorrespondencewithEP2.6.7.compliance
KitComponents
1vialwith1×108genomes(freeze-dried.)
3vialswithTrisbuffer(10mM,pH8.5)
AvailableSpecies
- Cat.No.52-0116Acholeplasmalaidlawii
- Cat.No.52-5571Bordetellapertussis
- Cat.No.52-0083Escherichiacoli
- Cat.No.52-0101Legionellapneumophila
- Cat.No.52-0129Mycoplasmaarginini
- Cat.No.52-0117Mycoplasmafermentans
- Cat.No.52-0115Mycoplasmagallisepticum
- Cat.No.52-0130Mycoplasmahyorhinis
- Cat.No.52-0112Mycoplasmaorale
- Cat.No.52-0119Mycoplasmapneumoniae
- Cat.No.52-0124Mycoplasmasynoviae
- Cat.No.52-0164Spiroplasmacitri
- Cat.No.52-0071Pseudomonasaeruginosa
Storage
Thekitmustbestoredat2-8°C(Shippedatambienttemperatures).