SwabUp™LabMonitoringPluskitwithacontamination-freePCRsystemisfortracingDNAcontaminationhot-spotsinmolecularBIOLOGylabsinordertoefficientlyeliminatethemandpreventfutureoccurrence.
Smallamountsofamplicon-ortargetDNAcontaminationscouldleadtoPCRartifactsandfalsepositiveresults.Originatingfromaerosolizedfragmentsincentrifuges,Pipettesandotherlabequipmentorfromsmallsplattersduringworkingwithopenreactiontubes,contaminantDNAisveryhardtoremoveandcanleadtocrosscontaminationsbetweensamplesandwidespreadproblemsthroughoutthetestingprocedureandinterpretationofresults.SwabUp™LabMonitoringPluskitcontainsasamplecollectionsystem,aDNAextractionsystem,whichwasoptimizedfortheefficientdetectionofthesmallestamountsofcontaminantDNA,andacontamination-freePCRSystem.
RecommendedUse/Scope
ThiskitisrecommendedfortheregularmonitoringofthelabworkareaanddetectionoftargetorampliconDNAcontaminations,butespeciallyforsevereandpersistentcasesofDNAcontamination,whereeverysinglereagentusedforPCRcouldpotentiallybecontaminatedmakingitextremelydifficulttotracethecontaminationsource.
SwabUp™LabMonitoringPluskitintroducesthecontamination-freeSwabUp™DNAmpMix,auniversalPCRmastermix,whichcontainsallPCRcorecomponents.Onlyreaction-specificprimersandtemplateDNAareaddedbytheuser.
SwabUp™LabMonitoringkitisforresearchuseonly.ItisnotrecommendedforclinicalanddiagnosticapplicationsorforthedetectionofRNAcontaminations.
KitComponents
Separatelypackedswabs,CollectionBuffertubes,spincolumn-basedDNAextractionsystem,SwabUp™DNAmpMix,RehydrationBuffer,MgCl2.
PackageSizes
- Cat.No.182-001010applications(25PCRreactions)
- Cat.No.182-005050applications(125PCRreactions)
RequiredConsumables
Ethanol(>96%abs.),1.5mlreactiontubes,PCRtubes
RequiredLabDevices
Microcentrifuge,heatblock,pipettingequipment,PCRThermocycler
ShelfLifeandStorage
DNAextractionsystemshouldbestoredatroomtemperature,CollectionBuffertubesmustbestoredat2–8°C.Swabscanbestoredat2–30°C.SwabUp™DNAmpMixshouldbestoredat2–8°C,andafterrehydrationat<-18°C.