ConviFlex™DNAmpMixisalyophilizedready-to-usePCRmastermix,whichconsistsofallcorecomponentsrequiredforPCRamplification,exceptDNAtemplateandprimers.
PCRamplificationhasbecomeanindispensablemethodineverymolecularBIOLOGylab.Usually,thecommonreactioncomponents(e.g.buffer,dNTPs,MgCl2,andDNApolymerase)aremixedtogetherbylaboperatorstocreateamastermix,whichisthenaliquotedtoindividualreactiontubes.Reaction-specificprimersandtemplateDNAareaddedpriortoPCR,addingfurtherpipettingstepstotheprocedure,andthusincreasingthemarginoferror,theriskofcross-contaminationsandworktime.TheapplicationofConviFlex™DNAmpMixrequiresminimalhands-ontimeandreducestheriskofcross-contaminationsduetotheminimalnumberofpipettingstepsrequiredtosetupthePCRreaction.
RecommendedUse/Scope
ConviFlex™DNAmpMixiscompatIBLewithbothconventionalPCRandqPCR,andissuitableforallregularPCRapplicationsincludingPCRassayswithcomplexgenomicorCDNAtemplates,lowcopynumbertargets,highspecificityPCR,T/Acloning,andmultiplexPCR.DNAtemplatecanbeextractedfromdiversestartingmaterialslikecellculturesupernatants,bacterialcolonies,andmammaliancellsandtissues.
ConviFlex™DNAmpMixisalsosuitableforPCRamplificationusingtemplateDNAdirectlyfromcellculturesupernatantsorbacterialsUSPensions.
KitComponents
ConviFlex™DNAmpMixcontainshotstartTaqpolymeraseanddNTPs(100reactions/vial),RehydrationBuffer,MgCl2(100mM)
PackageSizes
- Cat.No.191-0100100reactions
- Cat.No.191-0500500reactions
- Cat.No.191-10001000reactions
RequiredConsumables
Microcentrifugevials(1.5ml),Pipetteswithcorrespondingfiltertips,PCRtubes
RequiredLabDevices
Microcentrifuge,Heatblock,PCRThermocycler
ShelfLifeandStorage
LyophilizedConviFlex™DNAmpMixandRehydrationBuffershouldbestoredat2–8°C.Afterrehydration,ConviFlex™DNAmpMixshouldbestoredat<-18°Candisstableevenafterseveralfreeze/thawcycles.