Mynox®GoldrepresentsthefurtherdevelopmentoftheclassicalMynox®.Mynox®GoldisacombinationofastandardantibioticandtheBIOLOGicalMynox®reagent.Oneapplicationcomprisesofastartertreatmentthateliminatesmostofthemycoplasmaparticleswithoutharmingthecells.Themaintreatmentkillsallremainingmycoplasma.
Benefits
Nocytotoxicity–cytotoxicityisbasicallynotdetectableformostcelltypes.Mostcelllinesdonotshowanychangesinmorphologyduringtreatment.
Lowresistancerisk–duetothecombinationofanantibioticandareagentshowingabiophysicalmodeofactiondirectlyeliminatingthemycoplasma,formationofresistancesagainstMynox®Goldismostunlikely.
Highlyeffective–theratioforapermanenterADIcationiscloseto100%.
RecommendedUse/Scope
Mynox®Goldisintendedforresearchuseonly.Notapplicableforclinicaltreatment.NotapplicableforthedirecttreatmentofenvelopedvirusesorcellsharboringChlamydiaorotherbacteriaashostsystems.Theantibioticportionoftheproductmightaffecttheintegratedmicroorganisms.
KitComponents
1applicationofMynox®Goldcontains1vialforthestartertreatmentand3vialsforthemaintreatment.Eachvialcontains500µlasasterile,ready-to-usesolution.
PackageSizes
1applicationcanbeusedtocureonecellculturepermanentlyfrommycoplasmaburden.
- Cat.No.10-02012applications
Cat.No.10-05015applications
Cat.No.10-100110applications
ResultEvaluation
Eliminationsuccessshouldbeverifiedwithasuitablemycoplasmadetectionsystem,e.g.MinervaBiolabs’sVenor®GeMMycoplasmaPCRDetectionKits.
RequiredConsumables
6-welltissuecultureplateorsmalltissueculturepetridish(6mmØ)
Regularcellcultureplastics
RequiredLabDevices
Regularcellcultureequipment(incubator,centrifuge,Pipettes,etc.)
ShelfLifeandStorage
Kitcomponentsaremaintainableat+2to+8°Cforatleast6months.
Fig.CytotoxicityofMynox®Goldoncontaminatedcells.Adherentcelllines(Vero,ML/SMDBKandRK13)weretreatedwithMynox®Goldaccordingtotheprotocol4daysofincubation.ThecultureswereinactivatedandstainedasdescribedbyFlick&Gifford.Untreatedcultureshadbeenusedasreference(100%).