Venor®GeMClassicallowsfast,reliableandtime-savingroutinemonitoringofmycoplasmacontaminationbyPCR.
TypeofPCR
Forconventional,endpointPCR
RecommendedUse/Scope
ApplicableinresearchandindustryfordirecttestingofcellculturesandBIOLOGicals.Intendedforresearchuseonly.Notrecommendedforclinicaldiagnostics.
KitComponents
Lyophilizedprimersetsandnucleotides
10xreactionbufferoptimizedforMBTaqDNAPolymerase
LyophilizedpositivecontrolDNA
Lyophilizedinternalamplificationcontrol
PackageSizes
Primersetsandnucleotidesarepreparedinaliquotsof25tests.
- Cat.No.11-102525Tests
Cat.No.11-105050Tests
Cat.No.11-1100100Tests
Cat.No.11-1250250Tests
Resultevaluation
GelelectrophoresisatendpointofPCR
RequiredConsumables
Polymerase.Wehighlyrecommendourreliablehot-startMBTaqDNAPolymerase,Cat.-No.53-0050/100/200/250. PCRreactiontubes
OptionalforprocessvalidationandEP2.6.7complianttesting: InternalControlDNAextra(4vialsfor300µleachofinternalamplificationcontrol;Cat.No.11-1905) 10CFU™SensitivityStandardsavailableforallEPlistedmycoplasmaspecies
Requiredlabdevices
RegularPCRcycler
AgarosegelelectrophoresisandDNAstainingsystem
Pipettingequipment
Tubecentrifuge
ShelfLifeandStorage
Componentsaremaintainableat+2to+8°Cforatleast6months.Afterrehydratisationthereagentsmustbestoredat-18°C.
EP2.6.7compliance
UseofVenor®GeMClassicforQAtestingofbiologicalslikemasterandworkingcellbanks,autologouscells,culturemedia,bulkharvestandfinalproducttestingaccordingtoEP2.6.7isapplicableafterappropriatesamplepreparationandprocessvalidation.
Fig.AmplifiedPCRproductsarevisualizedbystandardgelelectrophoresis.